Journal: The American Journal of Tropical Medicine and Hygiene

Article Title: Evaluation of a Field-Portable DNA Microarray Platform and Nucleic Acid Amplification Strategies for the Detection of Arboviruses, Arthropods, and Bloodmeals

doi: 10.4269/ajtmh.2012.12-0048

Figure Lengend Snippet: ArboChip3.0 microarray detection and correct identification of NS5 PCR amplified laboratory preparations of mosquito-borne flaviviruses

Article Snippet: The oligonucleotide probes were synthesized directly on the ElectraSense 4x2K sectored microarray slides by CustomArray, Inc.

Techniques: Microarray, Amplification

Journal: The American Journal of Tropical Medicine and Hygiene

Article Title: Evaluation of a Field-Portable DNA Microarray Platform and Nucleic Acid Amplification Strategies for the Detection of Arboviruses, Arthropods, and Bloodmeals

doi: 10.4269/ajtmh.2012.12-0048

Figure Lengend Snippet: Comparison of amplification methods for microarray detection of cell culture and infected mosquito-derived virus. Virus abbreviations: DENV = dengue virus, WNV = West Nile virus, and TMUV = Tembusu virus.

Article Snippet: The oligonucleotide probes were synthesized directly on the ElectraSense 4x2K sectored microarray slides by CustomArray, Inc.

Techniques: Amplification, Microarray, Cell Culture, Infection, Derivative Assay

Journal: The American Journal of Tropical Medicine and Hygiene

Article Title: Evaluation of a Field-Portable DNA Microarray Platform and Nucleic Acid Amplification Strategies for the Detection of Arboviruses, Arthropods, and Bloodmeals

doi: 10.4269/ajtmh.2012.12-0048

Figure Lengend Snippet: Microarray flavivirus analysis of NS5 PCR amplified (A, B) cell culture-derived West Nile virus lineage 1 (WNV L-I) and (C, D) dengue virus type 4 (DENV-4) infected Aedes albopictus. (A, C) Average electrochemical detection (ECD) signals of flavivirus probes clustered into groups are used for first-level identification. Probes from positive groups are further categorized into subgroups. Analysis of the (B) WNV and (D) DENV subgroups are used to differentiate the viruses from other related species. Virus abbreviations: CYV = Chaoyang virus; DENV = dengue virus, EHV = Edge Hill virus; ILHV = Ilheus virus; JEV = Japanese encephalitis virus; MFV = mosquito-only flavivirus; MTBEV = mammalian tick-borne encephalitis virus; POTV = Potiskum virus; RBV = Rio Bravo virus; TMUV = Tembusu virus; ZIKV = Zika virus.

Article Snippet: The oligonucleotide probes were synthesized directly on the ElectraSense 4x2K sectored microarray slides by CustomArray, Inc.

Techniques: Microarray, Amplification, Cell Culture, Derivative Assay, Infection

Journal: The American Journal of Tropical Medicine and Hygiene

Article Title: Evaluation of a Field-Portable DNA Microarray Platform and Nucleic Acid Amplification Strategies for the Detection of Arboviruses, Arthropods, and Bloodmeals

doi: 10.4269/ajtmh.2012.12-0048

Figure Lengend Snippet: Microarray discrimination between the mosquito genera Aedes, Armigeres, Culex, and Mansonia of RT-rPCR amplified (A) Aedes albopictus and (B) Mansonia uniformis.

Article Snippet: The oligonucleotide probes were synthesized directly on the ElectraSense 4x2K sectored microarray slides by CustomArray, Inc.

Techniques: Microarray, Amplification

Journal: The American Journal of Tropical Medicine and Hygiene

Article Title: Evaluation of a Field-Portable DNA Microarray Platform and Nucleic Acid Amplification Strategies for the Detection of Arboviruses, Arthropods, and Bloodmeals

doi: 10.4269/ajtmh.2012.12-0048

Figure Lengend Snippet: Microarray bloodmeal analysis of Cytb polymerase chain reaction (PCR) amplified (A) chicken (Gallus gallus) whole blood and (B) pig (Sus scrofa domesticus) whole blood.

Article Snippet: The oligonucleotide probes were synthesized directly on the ElectraSense 4x2K sectored microarray slides by CustomArray, Inc.

Techniques: Microarray, Polymerase Chain Reaction, Amplification

Journal: The American Journal of Tropical Medicine and Hygiene

Article Title: Evaluation of a Field-Portable DNA Microarray Platform and Nucleic Acid Amplification Strategies for the Detection of Arboviruses, Arthropods, and Bloodmeals

doi: 10.4269/ajtmh.2012.12-0048

Figure Lengend Snippet: Field-identification of arboviruses, bloodmeals, and mosquito genera using the ArboChip3.0 in Lopburi and Kamphaeng Phet, Thailand, April to March, 2011

Article Snippet: The oligonucleotide probes were synthesized directly on the ElectraSense 4x2K sectored microarray slides by CustomArray, Inc.

Techniques: Microarray

Journal: The American Journal of Tropical Medicine and Hygiene

Article Title: Evaluation of a Field-Portable DNA Microarray Platform and Nucleic Acid Amplification Strategies for the Detection of Arboviruses, Arthropods, and Bloodmeals

doi: 10.4269/ajtmh.2012.12-0048

Figure Lengend Snippet: Field evaluations of the ArboChip3.0 DNA microarray platform using flavivirus-positive mosquito pools trapped in Lopburi (LB) and Kamphaeng Phet (KPP), Thailand, in March 2011. (A–C) Sample Th9-0011 contained a single blood-fed Culex quinquefasciatus mosquito. (D, E) Sample Th9-0122 contained a single blood-fed (bloodmeal not identified) Aedes aegypti mosquito. (F, G) Pool Th9-0164 contained 24 Culex tritaeniorhynchus mosquitoes. (A, D, F) Subgroup analysis of flavivirus non-structural protein 5 (NS5) reverse transcription-polymerase chain reaction (RT-PCR) amplicons positive for the groups mosquito-only flavivirus (MFV), dengue virus (DENV), and Japanese encephalitis virus (JEV), respectively. (B, G, E) Discrimination between the mosquito genera Aedes, Armigeres, Mansonia, and Culex of RT-rPCR amplicons. (C) Bloodmeal group analysis of mammalian Cytb PCR amplicons.

Article Snippet: The oligonucleotide probes were synthesized directly on the ElectraSense 4x2K sectored microarray slides by CustomArray, Inc.

Techniques: Microarray, Reverse Transcription Polymerase Chain Reaction